DEVELOPMENT AND VALIDATION OF REVERSED PHASE HPLC METHOD FOR ESTIMATION OF CEFTRIAXONE IN PHARMACEUTICAL DOSAGE FORM

  • RY Shah Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India
  • RK Jat Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India

Abstract

A simple, accurate rapid and precise RP-HPLC method has been developed and validated for determination of Ceftriaxone in bulk drug. The RP-HPLC separation was achieved on Promosil C-18, (250 mm, 4.6 mm, 5µm) using mobile phase buffer: methanol ph 6.8 (90: 10 v/v) at flow rate of 1.0 ml/min at ambient temperature. The retention times were 7.111 min. for Ceftriaxone. Calibration plots were linear over the concentration range 1-20µg/ml. Quantification was achieved with photodiode array detection at 260 nm over the concentration range of 1-50 µg/ml. The method was validated statistically and applied successfully for the determination of Ceftriaxone. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for the routine determination of Ceftriaxone in bulk drug.

Keywords    Ceftriaxone, Water, Buffer, Validation, HPLC.

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Author Biographies

RY Shah, Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India
Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India
RK Jat, Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India
Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India
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1.
Shah R, Jat R. DEVELOPMENT AND VALIDATION OF REVERSED PHASE HPLC METHOD FOR ESTIMATION OF CEFTRIAXONE IN PHARMACEUTICAL DOSAGE FORM. JDDT [Internet]. 15May2015 [cited 30Sep.2020];5(3):87-1. Available from: http://www.jddtonline.info/index.php/jddt/article/view/1152